9392122DNAreplicationreviewSauer.pdf

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Question Title: DNA Replication

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Name: _________________ DNA Replication Review Date: _________ Pd: ___ Part I. DNA Structure & Function 1. Draw a strand of DNA. Label the following... Adenine Thymine Cytosine Guanine Hydrogen bond Phosphate group Deoxyribose Nucleotide Nitrogen base 2. What type of organic compound is DNA? 3. What are the building blocks (monomers) of DNA called? 4. What are the sides of the “Twisted ladder” made of? What are the rungs (middle portion) made of? 5. Which nitrogen bases pair with one another? 6. DNA contains your genetic code, which is basically a long list of instructions for your cells, telling them what proteins to make, how these proteins interact, etc. You’ve made metaphors for cells—example: “the cell is a factory”. Using your knowledge of DNA, make a comparison of DNA to something that provides you with a list of instructions, or tells you how to make something. DNA is like: How? Part II. Chargaff’s Rules 7. Use Chargaff’s Rules to complete the table below. Cytosine Guanine DNA Adenine Thymine Sample 1 15% 35% 2 7% 3 2% 48% 8. The proportion of nitrogen bases are consistent within a species, but they do vary between different species. Use Chargaff’s base-pairing rules to complete the following table. Organism Percentage of each base Human Adenine Thymine Cytosine Guanine Cow 31 27 Salmon 22 Wheat 21 Yeast 19 9. The bar graph below shows the relative amount of bases (A & C) that are found in a particular segment of DNA. Draw bars that represent the relative amounts of the remaining bases, T & G. Part III. DNA Replication 10. DNA Replication takes place during ______________________ of Interphase, resulting in ___ DNA molecules. Each DNA molecule is made of ____ “old” (template) nucleotides and ___ “new” (complementary) nucleotides. 11. DNA is semiconservative because: a. Both RNA and DNA nucleotides are used. b. Part of the DNA is degraded/lost during replication c. Each new double helix contains one old and one new strand of nucleotides. d. Each new strand is identical–NOT complementary–to its template. 12. Draw a diagram showing a molecule of DNA undergoing replication. Draw one diagram for EACH of the 3 steps of DNA replication. a. Unwinding of the helix (enzyme = ____________________________) b. New strand is built using the template strand (enzyme = ____________________________) c. Sugar-phosphate backbone of the new strand is linked Using the following terms, please identify the structures above, numbered 1-4 and place the number next to the corresponding term below. ALSO pair the original nitrogen bases with their appropriate partnering base by filling in the boxes above. _____Template Strand _____DNA Polymerase _____Complementary Strand _____Helicase 13. If your DNA stopped replicating, what would happen to you? Please explain in detail. 14. Complete the template below with the complementary strand of nitrogen bases.